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Biomedical Imaging and Instrumentation

Biomedical Imaging and Instrumentation - Poster Session C

Poster F2 - Fasting induced lipid metabolic changes in drosophila intestines during aging

Friday, October 25, 2024

10:00 AM - 11:00 AM EST

Location: Exhibit Hall E, F & G

Presenting Author(s)

Yuhan Li

Research Assistant
UCSD
San Diego, United States

Co-Author(s)

Shuang Wu

Undergraduate Researcher
UCSD, California, United States
YL

Yajuan Li

Postdoctoral Researcher
University of California, San Diego, United States
SQ

Shuo Qin

Resistant Assistant
UCSD, United States
AW

Anna Wang

Resistant Assistant
UCSD
Eastvale, United States

Primary Investigator(s)

Lingyan Shi, PhD (she/her/hers)

Associate Professor
University of California, San Diego
La Jolla, California, United States

Introduction: Metabolism is essential for regulating energy use and nutrient processing in organisms, impacting growth, development, and aging. Within this complex system, lipid metabolism plays a crucial role in energy storage and cellular processes, and their regulation is key to maintaining metabolic balance. Intermittent fasting (IF), a diet pattern alternating between periods of fasting and eating, has gained attention for its potential to influence lipid metabolism and extend lifespan. Using drosophila models, our research focuses on how IF affects lipid metabolism in the gut and contributes to improved metabolic function and longevity.

Materials and

Methods: Drosophila flies were subjected to an adapted 3:1 diet, alternating between 3 days of fasting and 1 day of feeding until reaching 20 days of age, followed by a switch to normal feeding. At 35 days of age, flies were dissected, and gut images were collected using SRS imaging technology. These images were analyzed at the subcellular level, focusing on differences in lipid droplets between the control and fasting groups.

Results, Conclusions, and Discussions: Analysis revealed significant differences in lipid metabolism and turnover rates, as well as chemical components, between fasting and control groups. After fasting, an increased in the size and number of lipid droplets was observed in the fasted groups. Also, the lipid turnover rate on the lipid droplets was also increase in the fasted groups. In conclusion, fasting led to alterations in lipid metabolism pathways and turnover rates, suggesting a potential mechanism contributing to the observed increase in lifespan.

Acknowledgements (Optional):