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Biomaterials

Biomaterials - Poster Session C

Poster B6 - High Density Polyethylene Immersion in 10-Hydroxy-2-decenoic-acid Antimicrobial Properties for S. Aureus

Friday, October 25, 2024
10:00 AM - 11:00 AM EST
Location: Exhibit Hall E, F & G

Introduction:  High Density Polyethylene, or HDPE, is used to make medical consumables, food containers, and implants. HDPE is also prone to contamination by S. Aureus through attachment and formation of biofilm colonies. To demonstrate how HDPE may be protected from corruption, the polymer was immersed in 10-Hydroxy-2-decenoic-acid (10-H2DA), an antimicrobial saturated fatty acid found in Royal Jelly, at 10 mg of therapeutic to 1 mL of ethanol. We hypothesized that adsorbing 10-H2DA to the surface of HDPE would provide some antimicrobial activity against a common orthopaedic pathogen, S. aureus.

Materials and

Methods:  HDPE coupons were immersed in a solution of 10 mg of 10-H2DA to 1 mL of EtOH overnight. Video contact angle and FTIR were performed to characterize adsorption of 10-H2DA to the surface. For antimicrobial studies S. Aureus was grown overnight and then diluted 10 times and added to wells with coated HDPE and uncoated controls. After overnight incubation, planktonic growth was assessed using PrestoBlue viability reagent. Bacterial growth on coupons was also assessed with FilmTracer Live/Dead staining.

Results, Conclusions, and Discussions: Initial results in FTIR, contact angle, luminescent and florescent, as well as live/dead characterization analysis show that the 10-H2DA therapeutic was successful in surface functionalization of the HDPE, suggesting further use in antimicrobial biomedical applications. FilmTracer Live/Dead staining was also performed showing significant reduction of live bacteria after the therapeutic was applied. The FTIR and contact angle analysis showed that the 10-H2DA was indeed bound to the surface of the polymer with hydrophilic properties. Bacterial viability assays showed a significant reduction in S. aureus planktonic growth, likely due to a burst release of 10-H2DA (Figure 1). Bacterial biofilm was reduced, though not completely. The results of this study may indicate a role for coating with 10-H2DA as a short-term preventative measure for polyethylene implants and medical devices.

Acknowledgements (Optional):  NSF Award 1945094