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Biomedical Imaging and Instrumentation

Biomedical Imaging and Instrumentation - Poster Session E

Poster R5 - Differences in Super-paramagnetic Iron Oxide Labeling of Human Adipose Tissue and Bone Marrow Derived-Mesenchymal Stem Cells for MRI Cell Tracking

Saturday, October 26, 2024

10:00 AM - 11:00 AM EST

Location: Exhibit Hall E, F & G

Presenting Author(s)

AR

Anamika Roy

Graduate Student
FAMU-FSU College of Engineering and National High Magnetic Field Laboratory, Florida State University
Tallahassee, Florida, United States

Co-Author(s)

JB

Jamini Bhagu, MS

Graduate Student
FAMU-FSU College of Engineering and National High Magnetic Field Laboratory, Florida State University
Tallahassee, Florida, United States
DR

Dayna Richter, MS

Graduate Student
FAMU-FSU College of Engineering and National High Magnetic Field Laboratory, Florida State University
Tallahassee, Florida, United States

Primary Investigator(s)

Samuel C. Grant, PhD

Professor and Director, MRI Program
FAMU-FSU College of Engineering and National High Magnetic Field Laboratory, Florida State University
Tallahassee, Florida, United States

Introduction: Adipose-derived human mesenchymal stem cells (a-hMSC) and bone marrow-derived human mesenchymal stem cells (bm-hMSC) are widely used for therapeutic purposes across a plethora of diseases. Both cells have potential regenerative roles in tissue formation and angiogenesis as well as anti-inflammatory, homeostatic and immunoregulatory actions. This study seeks to evaluate the required dose of two different sizes of super-paramagnetic iron oxides (SPIO) particles to achieve MRI detectability using relaxation- and susceptibility-weighted contrast at high magnetic field, while assessing the biological impacts of SPIO.

Materials and

Methods: For this purpose, bm-hMSC and a-hMSC (P5) were cultured and incubated with ultra-small super-paramagnetic iron oxides (USPIO) (5-nm dia., Sigma-Aldrich) and micrometer-sized iron oxide (MPIO, 860-nm dia., Bangs Labs) for 12-18 h. Cytotoxic effects for different doses SPIO incubation were evaluated for each agent and cell type using cell count, fluorescent microscopy, MTT assays, colony forming assays and RT-PCR. On days 1, 3 and 7, the labeled cells underwent either induced osteogenic and adipogenic differentiation. The iron labeling efficiency was measured by Prussian blue staining, mass spectroscopy and T2* MRI analysis.

Results, Conclusions, and Discussions: As shown in Fig 1 by Prussian blue staining and T2*-weighted mapping (TR/TE=1000/10 ms) and quantified by mass spectroscopy (not shown), MPIO uptake was evident in both cell types despite being larger in size. At 3.33 mg/mL, MPIO saturated both cells. However, higher concentrations of MPIO (including 3.33 mg/mL) reduced cell count and increased cytotoxicity more than USPIO. As it is smaller in size, USPIO should have been internalized uniformly, but unlike bm-hMSC, labeling was not achieved for a-hMSC.

In conclusion, the study showed that there was a significant difference in iron labeling for a-hMSC and bm-hMSC depending on the size of the iron particles. This result requires future investigation of internalization of insoluble nanoparticles in stem cells.

Acknowledgements (Optional): This research is funded by the NIH (R01-NS102395 to SCG) and NSF (DMR-1644779) and State of Florida through the National High Magnetic Field Laboratory.