Poster 148 - The Development of the Scaffold Towards the In-Vitro Modeling of an Ischemic Stroke - Anwitha Kandula

Saturday, October 26, 2024

10:00 AM – 12:00 PM EST

Location: Exhibit Hall E, F & G

Presenting Author(s)

Anwitha Kandula, High School

Student Researcher
Clemson University Bioengineering
Simpsonville, South Carolina, United States

Co-Author(s)

Farhad Shokati, MSc (he/him/his)

Graduate Research Assistant
Clemson University
CENTRAL, South Carolina, United States
LA

Lidadi Agbomi (she/her/hers)

Graduate Research
Clemson University
Simpsonville, South Carolina, United States

Introduction: As elderly citizens throughout the U.S. continue to gain brain diseases, more people become at risk of an ischemic stroke. Developing a microfluidic chip by printing rigid 3D fibers of carbohydrate glass within a hydrogel scaffold, functions as a mold, mimicking vasculature. The scaffold will provide cell growth support and tissue formation. This scaffold will be a component of a microfluidic chip, which will assist in supporting patients' outcomes through clinical interventions and replicate the pathophysiology of an ischemic stroke.

Materials and

Methods: A flow chamber was fabricated and designed using biocompatible material through SOLIDWORKS software through Biomed Amber resign. Initially, gelatin was cast as the mold, which was compared with those originally from the literature, as its stiffness was determined using AFM - Atomic Force Microscopy. Next, the carbohydrate glass goes through preparation and then the dissolvable channels are printed with it. The GelMA-based hydrogel is prepared and the carbohydrate glass is dissolved with cell culture media.

Results, Conclusions, and Discussions: The preliminary tests involved its capability to mimic seepage-flow-like perfusion using a gelatin-filled chamber, creating a promising foundation in terms of the neuropathology for ischemic strokes. The scaffold is a component of the chip that can support the creation of structures that have significant support on tissue formation and cell growth. In general, the scaffold will provide short-term support to the cells as they grow new tissue

(Unapplicable at the moment of submission)

Acknowledgements (Optional): I would like to thank Dr. Bruce Gao, Farhad Shokati, and Lidadi Agbomi for their contributions and mentorship regarding this project. This research was supported by NSF SC EPSCoR (OIA-1655740).

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